Molecular Analysis (qPCR)

We provide comprehensive quantitative PCR (qPCR) services for the early detection and quantification of cyanobacteria (16S rRNA) and their associated toxin-producing genes. By analyzing the genetic potential of a bloom, we offer water managers a proactive lead time that traditional toxin analysis alone cannot provide.

Molecular Analysis (qPCR)

Standardized Testing (Phytoxigene Partnership): We have partnered with Phytoxigene to offer the CyanoDTec assay, recently approved as Standard Method 10120. This industry-leading technology provides precise quantification of total cyanobacteria and the specific genes responsible for microcystins, saxitoxins, cylindrospermopsins, and anatoxins/guanatoxin.

Custom In-House Capabilities: In addition to standardized kits, we utilize our own proprietary, in-house primer and probe sets. This allows us to offer flexible, specialized screening for emerging targets or unique environmental samples that require a more tailored molecular approach.

A "Total Bloom Profile": This service is designed to be complementary to our existing expertise. When paired with Algal Taxonomy (to identify the physical community) and Algal Toxin Analysis (to measure specific toxins or toxin classes), we provide the most complete assessment of bloom dynamics available.

Interpretation

Important Note on Interpretation: While qPCR identifies the genetic potential for toxin production, the presence of these genes does not provide an indication of actual toxin presence or concentration. To confirm if toxins are currently being produced and released, molecular data must be used in conjunction with Toxin Analysis (e.g., ELISA, LC-MS/MS), which remains the only way to verify and quantify active toxicity.